TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| S. gordonii DL-1 | Laboratory stock; 28 | |
| P. gingivalis | ||
| 33277 | Type strain from ATCC | Laboratory stock |
| SMF1 | Derivative of 33277 with an insertional inactivation of the mfa1 gene; Emr | 16 |
| cSMF1 | SMF1 containing pT-MFA, complemented strain; Emr Tcr | This study |
| KDP98 | Derivative of 33277 with an insertional inactivation of the fimA gene; Emr | 42 |
| E. coli | ||
| DH5α | F φ80dlacZΔ(lacZYA-argF)U169 endA1 supE44 recA1 relA1 | BRL |
| BL21(DE3)pLysS | Host for pET30 vector | Novagen |
| Plasmids | ||
| pET30 | pET expression vector | Novagen |
| pET-MFA | pET-30 with the mfa1 open reading frame | This study |
| pT-COW | Shuttle vector plasmid; Amr Tcr in E. coli; Tcr in P. gingivalis Mob+ Rep+ | N. Shoemaker |
| pT-MFA | pT-COW containing a 2.5-kb fragment containing the upstream and coding region of the mfa1 gene | This study |
a
Resistance to erythromycin (Emr), tetracycline (Tcr), and ampicillin (Amr) is indicated. Mob+, mobilizable; Rep+, ability to replicate in P. gingivalis.