TABLE 1.
Primer designation | Primer sequence | Targeta | Size of PCR product (bp) | Reference of source |
---|---|---|---|---|
chuA.1 | 5′-GACGAACCAACGGTCAGGAT-3′ | chuA | 279 | 11 |
chuA.2 | 5′-TGCCGCCAGTACCAAAGACA-3′ | |||
TspE4C2.1 | 5′-GAGTAATGTCGGGGCATTCA-3′ | tspE4.C2 | 152 | 11 |
TspE4C2.2 | 5′-CGCGCCAACAAAGTATTACG-3′ | |||
yjaA.1 | 5′-TGAAGTGTCAGGAGACGCTG-3′ | yjaA | 211 | 11 |
yjaA.2 | 5′-ATGGAGAATGCGTTCCTCAAC-3′ | |||
hly.1 | 5′-AGGTTCTTGGGCATGTATCCT-3′ | hlyC/A | 556 | 2 |
hly.2 | 5′-TTGCTTTGCAGACTGCAGTGT-3′ | |||
cnfl.1 | 5′-CAGTGACCGGATCTCCGTTAT-3′ | cnfl | 230 | 35 |
cnfl.2 | 5′-CGTGTAATTCTTCTGTACTTCC-3′ | |||
hra.1 | 5′-CAGAAAACAACCGGTATCAG-3′ | hra | 260 | 6 |
hra.2 | 5′-ACCAAGCATGATGTCATGAC-3′ | |||
papC.1 | 5′-GACGGCTGTACTGCAGGGTGTGGCG-3′ | papC | 328 | 33 |
papC.2 | 5′-ATATCCTTTCTGCAGGGATGCAATA-3′ | |||
papG.II.1 | 5′-GGGATGAGCGGGCCTTTGAT-3′ | papGII | 190 | 24 |
papG.II.2 | 5′-CGGGCCCCCAAGTAACTCG-3′ | |||
papG.III.1 | 5′-GGCCTGCAATGGATTTACCTGG-3′ | papGIII | 258 | 24 |
papG.III.2 | 5′-CCACCAAATGACCATGCCAGAC-3′ | |||
pheR.1 | 5′-GCCGCAATCTTAAGCAGTTG-3′ | pheU | 350 | 6 |
pheR.2 | 5′-GCACGACATTTCACGTCAGT-3′ | |||
pheR.1 | 5′-GCCGCAATCTTAAGCAGTTG-3′ | pheU (LR PCR) | 6 | |
yjgB.1 | 5′-ACCTTGCTCGCAGTTGATCT-3′ | leuX (yjgB) (LR PCR) | 6 | |
PheV.1 | 5′-AACCGGATTACGCATCTGTG-3′ | pheV (LR PCR) | This study |
LR PCR, long-range PCR with hra.1. C2, anonymous DNA fragment issued from subtractive hybridization (11).