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. 2005 Jul;73(7):4081–4087. doi: 10.1128/IAI.73.7.4081-4087.2005

TABLE 1.

Oligonucleotide primers used to amplify virulence-associated genes and for long-range PCR

Primer designation Primer sequence Targeta Size of PCR product (bp) Reference of source
chuA.1 5′-GACGAACCAACGGTCAGGAT-3′ chuA 279 11
chuA.2 5′-TGCCGCCAGTACCAAAGACA-3′
TspE4C2.1 5′-GAGTAATGTCGGGGCATTCA-3′ tspE4.C2 152 11
TspE4C2.2 5′-CGCGCCAACAAAGTATTACG-3′
yjaA.1 5′-TGAAGTGTCAGGAGACGCTG-3′ yjaA 211 11
yjaA.2 5′-ATGGAGAATGCGTTCCTCAAC-3′
hly.1 5′-AGGTTCTTGGGCATGTATCCT-3′ hlyC/A 556 2
hly.2 5′-TTGCTTTGCAGACTGCAGTGT-3′
cnfl.1 5′-CAGTGACCGGATCTCCGTTAT-3′ cnfl 230 35
cnfl.2 5′-CGTGTAATTCTTCTGTACTTCC-3′
hra.1 5′-CAGAAAACAACCGGTATCAG-3′ hra 260 6
hra.2 5′-ACCAAGCATGATGTCATGAC-3′
papC.1 5′-GACGGCTGTACTGCAGGGTGTGGCG-3′ papC 328 33
papC.2 5′-ATATCCTTTCTGCAGGGATGCAATA-3′
papG.II.1 5′-GGGATGAGCGGGCCTTTGAT-3′ papGII 190 24
papG.II.2 5′-CGGGCCCCCAAGTAACTCG-3′
papG.III.1 5′-GGCCTGCAATGGATTTACCTGG-3′ papGIII 258 24
papG.III.2 5′-CCACCAAATGACCATGCCAGAC-3′
pheR.1 5′-GCCGCAATCTTAAGCAGTTG-3′ pheU 350 6
pheR.2 5′-GCACGACATTTCACGTCAGT-3′
pheR.1 5′-GCCGCAATCTTAAGCAGTTG-3′ pheU (LR PCR) 6
yjgB.1 5′-ACCTTGCTCGCAGTTGATCT-3′ leuX (yjgB) (LR PCR) 6
PheV.1 5′-AACCGGATTACGCATCTGTG-3′ pheV (LR PCR) This study
a

LR PCR, long-range PCR with hra.1. C2, anonymous DNA fragment issued from subtractive hybridization (11).