TABLE 1.

Primer and probe sequences for amplification of IL-10, TGF-β, and Foxp3

Gene	Sequencea
HPRT	Forward, 5′-GCA GTA CAG CCC CAA AAT GG-3′
	Reverse, 5′-AAC AAA GTC TGG CCT GTA TCC AA-3′
	Probe, 5′-FAM-TAA GGT TGC AAG CTT GCT GGT GAA AAG GA-TAMRA-3′
IL-10	Forward, 5′-ACA ACA TAC TGC TAA CCG ACT CCT T-3′
	Reverse, 5′-AGG TAA AAC TGG ATC ATT TCC GAT A-3′
	Probe, 5′-FAM-TGG CAA CCC AAG TAA CCC TTA AAG TCC TG-TAMRA-3′
TGF-β	Forward, 5′-CCC CAC TGA TAC GCC TGA GT-3′
	Reverse, 5′-ACA AGA GCA GTG AGC GCT GA-3′
	Probe, 5′-FAM-TGA ACC AAG GAG ACG GA-TAMRA-3′
Foxp3	Forward, 5′-CCC AGG AAA GAC AGC AAC CTT-3′
	Reverse, 5′-TTC TCA CAA CCA GGC CAC TTG-3′
	Probe, 5′-FAM-ATC CTA CCC ACT GCT GGC AAA TGG AGT C-TAMRA-3′

^aFAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.