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Production and secretion of Fha44-HtrA and FHA-HtrA by B. pertussis. Twenty microliters of unconcentrated culture supernatant from B. pertussis BPSA85 (A and B), BPSA87 (A and B), or BPRA (C and D) or 20 μl of 10-fold-concentrated culture supernatant from BPSA199 (C and D) was analyzed by immunoblotting using anti-HtrA (A and C), anti-Fha44 (B), or anti-FHA (D) antibodies. The sizes of the molecular mass markers, expressed in kDa, are given on the right.
