TABLE 1.
Antileishmanial effect of cytokine antagonists in WT BALB/c micea
| Treatment | Liver parasite burden (LDU)
|
% Killing | |
|---|---|---|---|
| Day + 12 | Day + 21b | ||
| None | 876 ± 31 | 1,202 ± 60 | 0 |
| Anti-IL-10R | 315 ± 36** | 63 | |
| IgG | 1,245 ± 92 | 0 | |
| Anti-IL-4 | 1,319 ± 101 | 0 | |
| IgG | 1,141 ± 82 | 0 | |
| TGF-βIIR-Fc | 753 ± 53*** | 14 | |
| IgG | 1,327 ± 108 | 0 | |
| IL-13Rα2-Fc | 780 ± 87*** | 11 | |
| IgG | 1,458 ± 139 | 0 | |
a
12 days after infection, liver parasite burdens were determined, and mice then received (i) no treatment, (ii) a single day + 12 injection of anti-IL-10R or rat IgG (0.5 mg), anti-IL-4 MAb or rat IgG (5 mg) or TGF-βIIR-Fc or mouse IgG (4 mg/kg, ∼100 μg), or (iii) injections of IL-13Rα2-Fc or human IgG (0.2 mg)on days + 12, + 14, + 16, and + 18. Results are from two to four experiments and indicate mean ± SEM values for 6 to 15 mice per time point.
b
**, P < 0.05 versus day + 12 value. ***, P < 0.05 versus day + 21 value for corresponding IgG-treated control.