FIG. 7.

Schematic representation of LidA-mediated ER-derived vesicle recruitment to the L. pneumophila-containing vacuole. Upon L. pneumophila uptake by the host cell, LidA (stars) is injected into the host cell cytoplasm via the type IV secretion system (double lines). The protein then associates with the phagosomal membrane and with compartments of the secretory pathway, such as the ERGIC. In this model, LidA interacts with a host protein(s) of the secretory pathway to facilitate the recruitment of ER-derived vesicles to the L. pneumophila (Lp)-containing vacuole. This may be facilitated by the migration of the phagosome in the vicinity of the ERGIC and the Golgi apparatus.