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. 2005 Jul;73(7):3923–3928. doi: 10.1128/IAI.73.7.3923-3928.2005

FIG. 3.

FIG. 3.

Confocal laser-scanning micrograph of human pulmonary coccidioidal granulomata (scale bar as indicated; original magnification, ×40). The specimens were stained for IL-10 (red) and lymphocyte cell surface markers CD4, CD8, and CD20 (green). Arrows indicate the colocalization (overlay of red and green appears as yellow) of IL-10 and the respective surface maker, while arrowheads indicate the cytoplasmic (intracellular, red) presence of IL-10. IL-10 was produced by cells both in the clusters (A, C, E, and G) and in the mantle regions (B, D, F, and H). While both CD4+ (A and B) and CD20+ lymphocytes (C and D) express IL-10, CD8+ lymphocytes do not (E and F). Panel B depicts a cell expressing IL-10. The morphology and vesicular appearance suggested a macrophage. Panels G and H represent negative controls.