TABLE 2.
Predicted lipid A compositions of LOS preparation from O35E and O35EkdtA mutant based on MALDI-TOF MS analysis
| Source | Ion m/z
|
Predicted lipid A compositiona | Relative intensityb (%) | |
|---|---|---|---|---|
| Observed | Calculated | |||
| O35E | 2,028.30 | 2,029.19 | P2-PEA2-GlcNAc2-3-OHC12:04-C10:02-C12:01 | 24 |
| 1,905.35 | 1,906.18 | P2-PEA1-GlcNAc2-3-OHC12:04-C10:02-C12:01 | 100 | |
| 1,825.39 | 1,826.18 | P1-PEA1-GlcNAc2-3-OHC12:04-C10:02-C12:01 | 32 | |
| 1,782.34 | 1,783.17 | P2-GlcNAc2-3-OHC12:04-C10:02-C12:01 | 23 | |
| 1,751.20 | 1,752.04 | P2-PEA1-GlcNAc2-3-OHC12:04-C10:01-C12:01 | 29 | |
| O35EkdtA | 1,905.28 | 1,906.18 | P2-PEA1-GlcNAc2-3-OHC12:04-C10:02-C12:01 | 28 |
| 1,782.27 | 1,783.17 | P2-GlcNAc2-3-OHC12:04-C10:02-C12:01 | 100 | |
| 1,628.27 | 1,629.03 | P2-GlcNAc2-3-OHC12:04-C10:01-C12:01 | 29 | |
| 1,600.10 | 1,601.00 | P2-GlcNAc2-3-OHC12:04-C10:02 | 35 | |
| 1,568.99 | 1,569.87 | P2-PEA1-GlcNAc2-3-OHC12:04-C10:01 | 29 | |
| 1,456.14 | 1,456.89 | P2-GlcNAc2-3-OHC12:04-C12:01 (minus H2O) | 30 | |
| 1,445.97 | 1,446.86 | P2-GlcNAc2-3-OHC12:04-C10:01 | 52 | |
| 1,430.09 | 1,430.87 | P2-GlcNAc2-3-OHC12:03-C10:01-C12:01 | 27 | |
a
P, phosphate moieties; GlcNAc, N-acetylglucosamine; 3-OHC12:0, 3-hydroxydodecanoyl acyl chain; C12:0, dodecanoyl acyl chain; C10:0, decanoyl acyl chain.
b
The highest value of each strain represents 100% and others compared with the highest one.