FIG. 6.
Role of IL-10 in the enhancement of IL-12p40 production. C57BL/6 peritoneal macrophages (5 × 105 cells) were pretreated with a neutralizing MAb against IL-10 (10 μg/ml) or with its isotype control (IC) in the presence or absence of SB203580 (10 μM), U0126 (25 μM), or PD98059 (25 μM) for 2 h and then stimulated with rHagB (20 μg/ml) for 20 h. The levels of IL-10, IL-12p40, IFN-γ, and TNF-α in culture supernatants were determined by ELISA. Data are expressed as the mean of the increase in induction (n-fold) over unstimulated control ± standard error of the mean of six separate experiments. Values were significantly different when P was <0.05 (*), <0.01 (**), or <0.001 (***) in comparison to values for macrophages incubated with rHagB only.