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. 2024 Dec 30;15:10889. doi: 10.1038/s41467-024-55261-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 3 — a Flow cytometry analysis of the GFP positive ratio in the competition asssay. WT cells are mixed with non-targeting or ALCAM^-/- cells and infected with HAdV-C5/B7 or HAdV-C5 for 48 h. The infection rate of each chimeric viruses in ALCAM^-/- group is normalized to that in non-targeting sgRNA group. b Phylogenetic analyses of the clinical isolates of HAdV-B3 and -B7 using fiber gene as the reference. The clinical isolates used in this study are HAdV-B3 (GenBank: DQ099432.4), -B7 (GenBank: GQ478341.1), -B14 (GenBank: JQ824845) and -B35 (GenBank: AY128640.2), as highlighted. qPCR quantification of HAdV DNA levels in non-targeting sgRNA mock and ALCAM^-/- cells at 48 h post infection with clinical HAdV-B3 (c) and HAdV-B7 (d). e qPCR quantification of HAdV-B14 and -B35 L5 DNA levels in non-targeting sgRNA mock and ALCAM^-/- cells at 48 h post infection with HAdV-B14 and HAdV-B35 clinical isolates. f qPCR quantification of HAdV-B7 L5 DNA levels in non-targeting sgRNA mock, ALCAM^-/- and overexpression-rescued cells at 48 h post virus infection. g Representive images of the CPEs in non-targeting sgRNA mock, ALCAM^-/- and overexpression-rescued cells at 48 h post infection with clinical HAdV-B7. Scale bars, 100 μm. The experiment is repeated three times independently and similar results are obtained. h The effects of ALCAM overexpression on HAdV-C5/B7-GFP infection in Hela cells, as evaluated by flow cytometry quantification of GFP fluorescence. i qPCR quantification of viral L5 DNA levels in WT and ALCAM-overexprssed HeLa cells at 48 h post infection with clinical HAdV-B7. For (c–f and i), rplp0 is used as an internal control. For (a, c–f and h, i), data are presented as mean ± SD (n ≥ 3) from independent biological replicates. For (c), the p values between non-targeting sgRNA mock and ALCAM^-/- cell at an MOI of 0.01 and 0.1 are 1e-5 and 5e-5 respectively. The significant difference is analyzed by two-tailed unpaired Student’s t test. Source data are provided as a Source Data file.