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. 2005 Jul;49(7):3050–3052. doi: 10.1128/AAC.49.7.3050-3052.2005

FIG. 2.

FIG. 2.

Interaction of QnrA and ParE subunit does not depend on supercoiled plasmid DNA or ciprofloxacin. Reactions of 80 μl (40 μl per gel) were analyzed by native gel electrophoresis (A) and by Western blotting with Tag-100 antibody (QIAGEN) (B). Reaction mixtures contained 2 μM ParE subunit, 0.4 μM QnrA, 4.3 nM supercoiled pBR322 plasmid DNA, 18 μM ciprofloxacin, and 1 mM ADPNP where indicated in the figure. Prestained broad-range molecular weight markers (Bio-Rad) were run in lane 1 to allow comparison of the two gels and not for molecular sizing.