TABLE 1.
Bacterial strains and plasmids used in this study
| Strain | Plasmid | Relevant descriptiona | Reference and/or source |
|---|---|---|---|
| Escherichia coli | |||
| DH5α | Host strain for cloning | 4 | |
| S17-1 | Mobilizing strain for Bacteroides spp.; TMPr | 30 | |
| KAM3 | Deletion in the chromosomal acrAB genes from E. coli DH5α | 14 | |
| DH5α | pGERM | Suicide vector in Bacteroides; AMPrb ERYr | 27; N. B. Shoemaker |
| S17-1 | pFMB2744 | pGERM containing a 1,580-bp internal fragment from B. fragilis bmeB | This study |
| S17-1 | pBMA1 | pGERM containing a 828-bp internal fragment from B. fragilis bmeA | This study |
| S17-1 | pBOP3 | pGERM containing a 1,142-bp internal fragment from B. fragilis bmeC | This study |
| TOP10 | pCR-XL TOPO | Cloning vector; KANr | Invitrogenc |
| KAM3 | pTPOUT25 | pCR-XL-TOPO containing a 4,891-bp fragment from bmeAB in B. fragilis | This study |
| KAM3 | pTPIN5 | pCR-XL-TOPO containing a 2,622-bp fragment from bmeAB in B. fragilis | This study |
| Bacteroides fragilis | |||
| ATCC 25285 | Type strain | ATCC | |
| FMB271 | bmeB disruption mutant; ERYr | This study | |
| FMAB1 | bmeAB disruption mutant; ERYr | This study | |
| FMA1 | bmeA disruption mutant; ERYr | This study | |
| FOM1 | bmeC disruption mutant; ERYr | This study | |
| FMB1 | Homolog 1 disruption mutant; ERYr | This study |
a
Abbreviations: TMP, trimethoprim; AMP, ampicillin; ERY, erythromycin; KAN, kanamycin.
b
AMPr, ampicillin resistance expressed in E. coli only under aerobic conditions; ERYr, erythromycin resistance in Bacteroides spp.
c
Invitrogen Corp., Carlsbad, CA.