TABLE 1.
Stage-specific polyamine content of P. falciparum-infected RBCsa
| Cell | Polyamine concn (nmol 1010 cells−1)
|
||
|---|---|---|---|
| Putrescine | Spermidine | Spermine | |
| Rings | 35.9 ± 24.2 | 93.4 ± 62.9 | 5.1 ± 3.3 |
| Trophozoites | 454.5 ± 195.8 | 1,079.0 ± 185.0 | 39.8 ± 17.2 |
| Schizonts | 624.0 ± 227.7 | 1,295.4 ± 398.9 | 112.5 ± 53.2 |
| Cocultured noninfected RBCs | 23.1 ± 10.3 | 43.5 ± 19.6 | 11.9 ± 8.0 |
a
Prior to polyamine determination, infected RBCs were separated from noninfected RBCs by discontinuous Percoll-l-alanine gradient centrifugation to yield parasitemias >95%. The results represent the means ± standard deviations of eight independent determinations.