TABLE 5.
Composition of free sterols in total, plasma, and mitochondrial membranes from L. donovani WT and HePC-R promastigotes
| Sterol | RTa (h) | Mean % of total sterols ± SD (n = 3)b
|
|||||
|---|---|---|---|---|---|---|---|
| WT
|
HePC-R
|
||||||
| TM | PM | MM | TM | PM | MM | ||
| Cholesterol | 1.00 | 34.0 ± 2.7 | 25.1 ± 2.2 | 52.2 ± 4.8 | 58.8 ± 4.5 | 34.3 ± 3.1 | 30.8 ± 3.2 |
| 14α-Methylcholesta-5,7,24-trien-3β-ol | 1.02 | 0.2 ± 0.1 | 0.2 ± 0.1 | 0.3 ± 0.1 | 0.3 ± 0.1 | 0.2 ± 0.1 | 0.2 ± 0.1 |
| 14α-Methylcholesta-8,24-dien-3β-ol | 1.04 | 0.1 ± 0.1 | 0.2 ± 0.1 | 0.1 ± 0.1 | 0.2 ± 0.1 | 0.1 ± 0.1 | 0.1 ± 0.1 |
| Cholesta-5,7,24-trien-3β-ol | 1.07 | 0.2 ± 0.1 | 4.1 ± 0.5 | 0.3 ± 0.1 | 0.2 ± 0.1 | 0.3 ± 0.1 | 0.3 ± 0.1 |
| Ergosterol (ergosta-5,7,22-trien-3β-ol) | 1.10 | 24.9 ± 1.8 | 26.7 ± 1.8 | ND | 16.1 ± 1.5 | 26.5 ± 2.2 | 33.0 ± 3.2 |
| Ergosta-5,7,22,24(24′)-tetraen-3β-ol | 1.13 | 1.8 ± 0.1 | 1.6 ± 0.2 | 0.9 ± 0.1 | 0.6 ± 0.1 | 1.3 ± 0.1 | 4.1 ± 0.4 |
| Ergosta-5,7,24(24′)-trien-3β-ol | 1.17 | 24.5 ± 2.2 | 22.4 ± 2.1 | ND | 9.2 ± 1.1 | 20.5 ± 0.3 | 4.0 ± 0.4 |
| Stigmasta-7,24(24′)-dien-3β-ol | 1.18 | ND | 3.9 ± 0.3 | 6.1 ± 0.6 | ND | 6.1 ± 0.5 | 3.1 ± 0.4 |
| Ergosta-7,22-dien-3β-ol | 1.19 | ND | 3.1 ± 0.4 | 5.8 ± 0.6 | ND | 1.1 ± 0.1 | 21.7 ± 1.8 |
| Ergosta-7,24(24′)-dien-3β-ol | 1.20 | 5.7 ± 0.6 | 7.2 ± 0.7 | 0.6 ± 0.1 | 1.9 ± 0.2 | 3.4 ± 0.3 | 0.5 ± 0.1 |
| 14α-Methylergosta-5,7,24(24′)-trien-3β-ol | 1.21 | 0.2 ± 0.1 | 0.3 ± 0.1 | 0.1 ± 0.1 | 0.1 ± 0.1 | 0.1 ± 0.1 | 0.2 ± 0.1 |
| 4,4α-Dimethylcholesta-8,24-dien-3β-ol | 1.22 | 0.3 ± 0.1 | 0.3 ± 0.1 | 0.2 ± 0.2 | 0.3 ± 0.1 | 0.2 ± 0.1 | 0.2 ± 0.1 |
| β-Sitosterol (stigmast-5-en-3β-ol) | 1.225 | ND | 2.5 ± 0.3 | 26.1 ± 2.2 | ND | 1.1 ± 0.1 | ND |
| 4α,14α-Dimethylcholesta-8,24-dien-3β-ol | 1.23 | 0.2 ± 0.1 | 0.1 ± 0.1 | 0.2 ± 0.1 | 0.1 ± 0.1 | 0.1 ± 0.1 | 0.2 ± 0.1 |
| 14α-Methylergosta-8,24(24′)-dien-3β-ol | 1.25 | 0.2 ± 0.1 | 0.2 ± 0.1 | 0.1 ± 0.1 | 0.2 ± 0.1 | 0.2 ± 0.1 | 0.3 ± 0.1 |
| 4,4α-Dimethylergosta-8,24(24′)-dien-3β-ol | 1.27 | 0.8 ± 0.1 | 0.6 ± 0.1 | 0.4 ± 0.1 | 0.8 ± 0.1 | 0.1 ± 0.1 | 0.3 ± 0.3 |
| 4α,14α-Dimethylergosta-8,24(24′)-dien-3β-ol | 1.28 | 0.4 ± 0.1 | 0.3 ± 0.1 | 0.3 ± 0.1 | 0.2 ± 0.1 | 0.2 ± 0.1 | 0.2 ± 0.1 |
| 24-Methylenedihydrolanosterol | 1.45 | 1.7 ± 0.1 | 1.1 ± 0.1 | 0.8 ± 0.1 | 1.1 ± 0.1 | 0.7 ± 0.1 | 0.6 ± 0.1 |
| Total C-24-alkylated sterols | 60.2 | 66.8 | 35.4 | 30.2 | 60.2 | 46.3 | |
| Other sterols (not identified) | 4.8 | 0.1 | 5.5 | 9.9 | 3.5 | 0.2 | |
a
RT, retention time relative to that of cholesterol in gas chromatography.
b
TM, total membrane; PM, plasma membrane; MM, mitochondrial membrane; ND, not detected.