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Expression of AAC(6′)-Ib and mutant derivatives. Total proteins obtained from E. coli TOP10 cells harboring either pBADMW1 (MW1) (wild type), pBADD117A (D117A substitution), or pBADL120A (L120A substitution) induced in the presence of 0.2% arabinose for 4 h were separated by 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The sizes of the molecular mass standards are shown to the left, and the position of AAC(6′)-Ib is shown to the right.
