TABLE 1.
Plasmid (substitution)a | Mean acetylation ± SD (cpm/μg protein)b
|
|
---|---|---|
AMK | KAN | |
pBADMW1 (no substitution) | 13,632 ± 95 | 12,989 ± 440 |
pBADD117A (D117A) | 10 ± 3 | 5 ± 2 |
pBADL120A (L120A) | 1,633 ± 38 | 5,190 ± 357 |
Soluble extracts were obtained from E. coli TOP10 harboring the indicated plasmid.
Values are averages of triplicates.