TABLE 1.
gyrA mutations and fluoroquinolone susceptibility of Mycobacterium tuberculosis complex strains
| Strain no. (designation) | Amino acid change | Allele | MIC (μg/ml) of:
|
|||||
|---|---|---|---|---|---|---|---|---|
| OFL (R/S)a | CIP | LEV | SPX | GAT | MOX | |||
| 1 (MTB H37Rv) | None | WT | 1 (S) | 1 | 0.125 | 0.125 | 0.06 | 0.125 |
| 2 (MTB P10) | None | WT | 1 (S) | 0.5 | 0.125 | 0.125 | 0.125 | 0.125 |
| 3 (MTB Ru108) | None | WT | 2 (S) | 1 | 1 | 0.5 | 0.5 | 1 |
| 4 (MTB Ru108-6) | None | WT | 2 (S) | 1 | 1 | 0.5 | 0.5 | 1 |
| 5 (MTB Ru124) | S95T | AGC95ACC | 1 (S) | 0.5 | 0.5 | 0.5 | 0.125 | 0.125 |
| 6 (MTB Ru95-0) | S95T | AGC95ACC | 0.5 (S) | 0.5 | 0.25 | 0.5 | 0.125 | 0.125 |
| 7 (MTB Ru95-3) | S95T | AGC95ACC | 0.5 (S) | 0.5 | 0.25 | 0.5 | 0.06 | 0.125 |
| 8 (MTB F16) | S95T | AGC95ACC | 0.5 (S) | 0.5 | 0.25 | 0.5 | 0.06 | 0.125 |
| 9 (MTB M23) | S95T | AGC95ACC | 2 (S) | 2 | 1 | 2 | 0.25 | 0.5 |
| 10 (MTB R21) | S95T | AGC95ACC | 16 (R) | 8 | 8 | 8 | 2 | 4 |
| 11 (MTB Ru152) | S95T | AGC95ACC | 8 (R) | 8 | 4 | 8 | 1 | 2 |
| 12 (MTB R24) | A90V | GCG90GTG | 8 (R) | 8 | 8 | 4 | 2 | 4 |
| 13 (MTB A1) | A90V | GCG90GTG | 8 (R) | 4 | 4 | 2 | 1 | 1 |
| 14 (MTB A3) | A90V | GCG90GTG | 8 (R) | 4 | 4 | 2 | 1 | 2 |
| 15 (MTB R19) | A90V-S95T | GCG90GTG-AGC95ACC | 4 (R) | 8 | 4 | 2 | 2 | 2 |
| 16 (MTB F9) | A90V-S95T | GCG90GTG-AGC95ACC | 4 (R) | 4 | 4 | 2 | 1 | 1 |
| 17 (MTB F11) | A90V-S95T | GCG90GTG-AGC95ACC | 8 (R) | 4 | 2 | 2 | 1 | 1 |
| 18 (MTB F18) | A90V-S95T | GCG90GTG-AGC95ACC | 8 (R) | 8 | 4 | 4 | 1 | 1 |
| 19 (MTB Ru95-8) | A90V-S95T | GCG90GTG-AGC95ACC | 8 (R) | 4 | 4 | 2 | 0.5 | 1 |
| 20 (MTB Ru95-9) | A90V-S95T | GCG90GTG-AGC95ACC | 8 (R) | 4 | 4 | 2 | 1 | 1 |
| 21 (MTB M6159) | S91P | TCG91CCG | 8 (R) | 4 | 4 | 2 | 1 | 2 |
| 22 (MTB M60) | S91P-S95T | TCG91CCG-AGC95ACC | 4 (R) | 4 | 2 | 1 | 0.5 | 1 |
| 23 (MTB R1) | D94A | GAC94GCC | 4 (R) | 4 | 4 | 2 | 0.5 | 1 |
| 24 (MTB MO) | D94N | GAC94AAC | 8 (R) | 4 | 2 | 2 | 1 | 1 |
| 25 (MTB F7) | D94G-S95T | GAC94GGC-AGC95ACC | 8 (R) | 4 | 4 | 4 | 2 | 4 |
| 26 (MTB F15) | D94G-S95T | GAC94GGC-AGC95ACC | 8 (R) | 4 | 4 | 2 | 1 | 2 |
| 27 (MTB M3) | D94G-S95T | GAC94GGC-AGC95ACC | 8 (R) | 4 | 2 | 2 | 0.5 | 1 |
| 28 (MTB Ru227-15) | D94G-S95T | GAC94GGC-AGC95ACC | 8 (R) | 4 | 4 | 2 | 1 | 1 |
| 29 (BCG HETb) | D94H-S95T | GAC94CAC-AGC95ACC | NDc | ND | ND | ND | ND | ND |
a
R, resistant; S, susceptible. The strains were considered R or S to OFL if the MIC in Middlebrook 7H11 agar was >2 μg/ml or ≤2 μg/ml of OFL, respectively (18).
b
BCG HET refers to DNA from a BCG strain received by Howard E. Takiff.
c
ND, not determined.