FIG. 2.

Effect of roxithromycin (ROX) on p47-phox and p67-phox translocation. PMN were preincubated for 30 min with 0.3% DMSO or roxithromycin. Membrane and cytosol fractions were then prepared, and p47-phox and p67-phox were detected as described in the text. All samples contained the same quantity (10 μg) of protein. (A) PMN were preincubated for 30 min with 0.3% DMSO or roxithromycin (25 to 100 mg/liter). One experiment representative of three different experiments is shown. (B) PMN were preincubated for 30 min with 0.3% DMSO (ROX −) or 100 mg/liter roxithromycin (ROX +). They were further stimulated with PMA at 1 μg/ml for 8 min (PMA +) or left unstimulated (PMA −). Two experiments representative of four different experiments are shown. (C) PMN were preincubated for 30 min with 0.3% DMSO (control; lanes marked ROX −) or 100 mg/liter roxithromycin (lanes marked ROX +). They were further stimulated with fMLP (10⁻⁶ M, 1 min). One experiment representative of four different experiments is shown.