Fig. 3.

Daidzein alleviates lovastatin-induced skeletal muscle atrophy in mice. The mice were evenly divided as follow (n = 8). After treatment with LV (lovastatin, 80 mg/kg/day), DA (daidzein, 150 mg/kg/day), and LV (lovastatin, 80 mg/kg/day) combined with DA (L, M, H)-(daidzein, 50,100,150 mg/kg/day) for 2 months, the mice were detected. A The mice body weight. B Weight of gastrocnemius muscle. C Grip strength. D Hematoxylin–eosin staining of mice gastrocnemius muscle (scale bar = 50 µm). E Serum CK activity. F Extract protein from gastrocnemius muscle of mice in each group and immunoblotting was used to test the protein expression levels. The image J was used to quantify the protein level. The mean ± SEM was used to present the data. *p < 0.05; **p < 0.01; ***p < 0.001, (n = 3)