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HPLC radiochromatogram of intracellular extracts from PBM cells incubated with 10 μM [3H]-d-FDOC for 24 h. Metabolites were separated by reversed-phase HPLC with a Columbus 5-μm C18 column (Phenomenex) using a model Pro Star (Varian) with manual injection. The mobile phase consisted of buffer A (25 mM ammonium acetate with 5 mM TBAP [pH 7.0]) and buffer B (methanol). Elution was performed by using a multistage linear gradient of buffer B.
