Fig. 5.

CD4⁺ T/CD8⁺ T/NK cells is involved in the anti-tumor effect of RTRT and CNRT. A Schematic diagram of CD4⁺ T/CD8⁺ T/NK cells antagonist mouse model construction. B, E, H, K tumor growth curves of control, RTRT, and CNRT of mice with or without antagonistic CD4⁺ T/CD8⁺ T/NK cells. n ≥ 4. ^*, P < 0.05. ^**, P < 0.01. ^***, P < 0.001. ns indicates no statistical difference. The statistical test was performed using Two-way ANOVA, followed by Tukey's multiple comparisons test. C, F, I, L Kaplan–Meier survivalcurves were generated of control, RTRT, and RTRT of mice with or without antagonistic CD4⁺ T/CD8⁺ T/NK cells, and significance was determined by log-rank (Mantel-Cox) test. ^*P < 0.05. D, G, J, M Physical images of lung metastases in each group of mice in the CD4⁺ T/CD8⁺ T/NK cells antagonist model (Left). Quantitative analysis of lung metastasis in each group of mice in the CD4⁺ T/CD8⁺ T/NK cells antagonistic model (Right). n ≥ 3. ^*, P < 0.05. ^**, P < 0.01. ^***, P < 0.001. ns indicates no statistical difference. The statistical test was performed using One-way ANOVA, followed by Tukey's multiple comparisons test