Fig. 7.

RBM47 could regulate PDIA6 mRNA stability by binding to its 3′-UTR region. A The mRNA level of PDIA6 expression in PC cells (normalized by GAPDH). B Western blot for PDIA6 expression. C Quantification of the protein level of PDIA6 expression in B. D RNA-binding protein immunoprecipitation (RIP) assay for verifying the interaction between RBM47 and the PDIA6 mRNA. E The mRNA level of RBM47 expression in 293 T cells (normalized by GAPDH). F The schematic diagram of the binding between RBM47 and the PDIA6 mRNA. Dual luciferase reporter assay for determining the effect of RBM47 overexpression on relative luciferase activity (firefly/renilla) of the 3′-UTR sequences of the PDIA6 mRNA. G The schematic diagram and percentage of remaining PDIA6 mRNA in PC cells after Actinomycin D treatment (normalized by GAPDH). For Fig. 7A-C, *p < 0.05, **p < 0.01 versus EV^Dox− or Control; ^##p < 0.01 versus EV, OE-RBM47^Dox− or shRBM47^Dox− For F, G, **p < 0.01 versus EV or OE-RBM47^Dox−