TABLE 2.
Deduced amino acid sequence of the phage-displayed peptides and their antibacterial activities against R2866
| Clone | Frequencya | Amino acid sequence | pIb | No. of virions/μl (concn [μM])c
|
|
|---|---|---|---|---|---|
| MICd | MBC99.5e | ||||
| hi3/17 | 5/15 | KQRTSIRATEGCLPS | 9.50 | 2 × 109 (0.8) | 3 × 109 (1.2) |
| hi3/2 | 1/15 | GRHRTSVPTDEVFIT | 6.75 | 1 × 1010 (4.1) | 1.5 × 1010 (6.2) |
| hi3/12 | 1/15 | KKSHHPSSEWGLNLT | 8.61 | 1 × 1010 (4.1) | 2 × 1010 (8.25) |
| hi3/11 | 1/15 | KQRDSRSGYTAPTLV | 9.99 | 1 × 1010 (4.1) | 2 × 1010 (8.25) |
| hi3/13 | 1/15 | RNHGTDRATTIPPLS | 9.61 | 2.3 × 1010 (9.4) | 2.3 × 1010 (9.4) |
a
That is, the frequency of the phage clone isolated from the f88-4/15-mer library.
b
pI, calculated isoelectric point.
c
The molar concentration of phage peptides assuming that all 150 copies displayed on pVIII are antimicrobial is given in parentheses.
d
MIC is defined as the minimum concentration of the phage-displayed peptides resulting in no detectable growth.
e
MBC99.5 is defined as the lowest concentration of phage-displayed peptides necessary to kill 99.5% of the bacteria.