TABLE 2.
Tn1546 types found among E. faecium clinical isolates recovered at three different Portuguese hospitals (1996 to 2003)a
| Type | PCR product for specific primer pair (annealing sites)
|
No. of isolates | Dateb | City(ies) | PFGE type(s)c | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| p1p2 (22-1330) | p3p4 (1222-2353) | p5p6 (2227-3525) | p7p8 (2769-4042) | p9p10 (3569-4793) | p11p12 (4675-6353) | p13p14 (6229-8021) | p15p16 (6979-8920) | p17p18 (8889-10473) | p19p1 (10403-10830) | |||||
| A | + | + | + | + | + | + | + | + | + | + | 2 | 04/98-10/00 | Coimbra | 92, 98 |
| D | − | + | + | + | + | + | + | + | + | + | 1 | 06/02 | Porto | 76 |
| X | − | − | − | − | − | + | + | − | − | + | 1 | 03/00 | Coimbra | 84 |
| PP-2 | + | + | + | + | + | + | + | + | − | + | 7 | 10/01-10/02 | Coimbra, Viseu | (70), 92, 93 |
| PP-3 | + | + | + | + | + | + | + | + | − | − | 1 | 03/02 | Coimbra | 94 |
| PP-4 | + | + | + | + | + | + | + | + | ++c | + | 11 | ?/97-04/02 | Coimbra | 76, 78, 80, 82, 88, 90, 95, 97, 108, 112, 122 |
| PP-5 | + | + | + | + | + | + | + | + | ++c | − | 15 | 09/02-03/03 | Coimbra, Viseu, Porto | 70, 71, 78, 99, 100, 72, 103, 104 |
| PP-9 | + | − | − | + | + | + | + | + | ++ | + | 2 | 01/00 | Coimbra | 74, 106 |
| PP-10 | − | + | + | + | + | + | + | − | + | + | 1 | ?/96 | Coimbra | 83 |
| PP-13 | − | − | − | − | + | + | + | − | − | + | 6 | 01-12-2002 | Porto | (78), 86, 88, 91, 119 |
| PP-15 | − | − | − | − | + | + | + | + | + | + | 1 | ?/01 | Porto | 96 |
| PP-16 | − | − | − | − | − | + | + | + | + | + | 1 | 06/01 | Porto | 77 |
| PP-20 | − | − | − | − | − | − | + | − | − | + | 1 | ?/01 | Porto | 85 |
| PP-23 | − | − | + | − | − | + | + | − | − | + | 1 | 09/02 | Porto | 119 |
| PP-24 | − | + | + | + | + | + | + | − | ++c | − | 1 | 03/03 | Coimbra | ND |
| PP-25 | − | − | − | − | + | + | + | − | + | + | 1 | 07/01 | Porto | 88 |
| PP-27 | + | + | + | + | + | + | − | − | + | − | 1 | 03/02 | Coimbra | 87 |
Tn1546 types were designed according to the Woodford scheme (32). For those that did not have a specific previously described type, we used our own designation (PP from Portugal-Oporto followed by a number randomly chosen). +, amplification; −, no amplification; ++, amplification of sequences larger than those of the expected size.
See footnote b of Table 1.
Positive amplification using ISEf1-F (5′-GGT GTT ACG ATG TCT GAA ATT GC-3′) and p18. The clones in which vancomycin resistance was transferred by conjugation appear underlined. Variable Tn1546 transference to receptor strains among isolates belonging to the same PFGE appear in parenthesis. PFGE types identified in more than one hospital appear in bold.