TABLE 2.
Vector mobilization by transfection of an env deletion mutant of HIVa
| Vector 293T cells were transduced with | Vector titer (GFU/ml) with:
|
|
|---|---|---|
| Rev/MS-C | None | |
| HIV-CL-CG | 4.05 × 103 ± 9.19 × 102 | 7.35 × 103 ± 3.32 × 103 |
| HIV-CL-CG-MS | 3.45 × 103 ± 9.19 × 102 | <1 |
| HIV-CS-CG | <1 | <1 |
| HIV-CS-CG-MS | <1 | <1 |
293T cells were transduced with the indicated vectors and transfected with an HIV-1 env deletion mutant (5) of pNL4-3, pHIT-G, and with or without Rev/MS-C fusion protein. Vector titers in the supernatant were determined 2 days after transfection. The means of three independent experiments and the standard deviations are shown. GFU, GFP-forming units.