Figure 2.

For the initial compound screening, SK-MEL-5 cells were treated with 1 uM and 0.1 uM of TYR targeting INTASYL compounds 1–36 for 48 hours. mRNA levels were assessed by qPCR and plotted relative to the non-targeting control (NTC). Hits were identified as those compounds with silencing of greater than 50%. These compounds were chosen for further chemical optimization.