FIG. 3.

Immunogenicity of CMV/R plasmid DNA vaccines expressing HIV-1 Gag-Pol-Nef in mice. Mice (n = 8/group) were immunized with 50 μg of the parental 1012 DNA vaccine or the CMV/R DNA vaccine expressing HIV-1 Gag-Pol-Nef at weeks 0 and 6. Splenocytes were assessed for Gag-, Pol-, and Nef-specific cellular immune responses by IFN-γ ELISPOT assays 3 weeks after the (A) initial and (B) week 6 boost immunizations. (C) Splenocytes depleted of CD8⁺ T lymphocytes were also assessed in similar ELISPOT assays following the boost immunization.