Fig. 6.

αhiPSC-CM specific hPBMCs contribute to myocarditis phenotype.

A) Viability assay utilizing hiPSC-CM antigen exPBMCs at 72 h. See methods for exPBMC culture protocol details. ExPBMCs demonstrate increased cell mediated cytotoxicity compared to non-exPBMCs. Technical replicates indicated on graph, CM Control (n = 8), Quiescent PBMC Isotype (n = 9), Quiescent PBMC Checkpoint (n = 10), hPBMCs + IL2/15 (n = 12), Ex-PBMC Isotype (n = 12), Ex-PBMC Checkpoint (n = 12), Activated Ex-PBMC (n = 12).

B) Phalloidin stain of murine colon cancer CT26 line (i and ii) and HEK293T cell line (iii and iv). Control lines cultured in isolation (i and iii) as well as cell lines cultured with activated hPBMCs and ICI mAbs (ii and iv) are shown. Imaging performed at 72 h. Experiment performed once.

Tukey's multiple comparisons test for (A). Experiments performed twice.