FIG. 2.
Schematic representation of the DNA sequence arrangement of the wild-type HSV-1 and HCMV genome and the recombinant HSV-1 viruses used in this study. Both HCMV (line 2) and HSV-1 (line 6) have group E genomes characterized by two covalently linked components, L and S, each composed of unique sequences (UL and US) flanked by inverted repeat sequences. The locations of the HCMV IRS1, IRS263, and TRS1 genes in the wild-type HCMV genome are shown (line 1). The HCMV IE2 gene and the location of the in-frame deletion mutation in IE2 86 exon 5 (Δ) is also shown (line 3). Line 4 demonstrates the location of one of the two copies of the HSV-1 γ134.5 gene. In the Δγ134.5 parent virus, R3616, both copies of the γ134.5 gene have been deleted, as represented in line 5. Line 7 represents the UL3, UL4 genetic domain in the wild-type and R3616 genome. Line 9 shows the UL3, UL4 domain of the recombinant virus C101, derived from R3616, containing the CMV immediate early promoter-driven EGFP gene. The recombinant virus C130, represented in line 11, contains the HCMV TRS1 gene under control of the HCMV IE promoter in the UL3, UL4 intergenic region of a Δγ134.5 virus. The Δγ134.5 recombinant C132 (expressing IRS1 transcript but not IRS1 protein) and C134 are represented in line 13 and 15, respectively. They contain the CMV IE promoter and HCMV IRS1 gene in the UL3, UL4 intergenic region. Lines 8, 10, 12, 14, and 16 represent the predicted fragments produced by PstI restriction digestion of the viral DNAs. The repair viruses C131 and C135 are not included but are predicted to be identical schematically to the C101 virus (line 9). P, PstI.