TABLE 2.
Comparative neutralization of Env-pseudotyped and PBMC-grown HIV-1
| Animal | Neutralization of Env-pseudotyped or PBMC-grown HIV-1 by sera from SOSIP.R6 gp140-immunized rabbitsa
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Env pseudotyped HIV-1
|
PBMC-grown HIV-1
|
|||||||||||
| JR-FL
|
MN
|
JR-FL
|
MN
|
|||||||||
| 50% | 70% | 90% | 50% | 70% | 90% | 50% | 70% | 90% | 50% | 70% | 90% | |
| 222 | — | — | — | >160 | 111 | 26 | — | — | — | 14 | — | — |
| 223 | — | — | — | >160 | 74 | 20 | — | — | — | — | — | — |
| 224 | 18 | — | — | >160 | 65 | 18 | — | — | — | 18 | — | — |
| 228 | 33 | 10 | — | >160 | >160 | >160 | — | — | — | 37 | 26 | 14 |
| 229 | 17 | — | — | >160 | >160 | 50 | — | — | — | 34 | 22 | 11 |
| 234 | 10 | — | — | >160 | >160 | 45 | — | — | — | 24 | 12 | — |
| 236 | 19 | — | — | >160 | >160 | >160 | — | — | — | 154 | 100 | 45 |
| 238 | 38 | 15 | — | >160 | >160 | 59 | — | — | — | 35 | 21 | — |
| 240 | 29 | — | — | >160 | >160 | >160 | — | — | — | 103 | 67 | 29 |
| 241 | >160 | >160 | 24 | 125 | 75 | 26 | 101 | 56 | — | — | — | — |
a
The reciprocal of the dilution which resulted in 50%, 70%, or 90% neutralization of infectivity. Neutralization of Env-pseudotyped HIV-1 was determined on coreceptor-bearing U87.CD4 cells, while neutralization of PBMC-grown HIV-1 was determined on PBMC. A dash indicates that <50% neutralization was observed at the 1:10 dilution.