TABLE 3.
Assessment of cross-neutralization activity in the ViroLogics Inc. Phenosense assay
| Animal or control | Time serum sample obtained (wk) | Neutralization of Env-pseudotyped HIV-1 by rabbit seraa
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| SF162 | NL4/3 | BaL | 1196 | JR-FL | JR-CSF | 1168 | MLV | ||
| 5695-4 | 0 | — | — | — | — | — | — | — | — |
| 20 | 4,658 | 90 | 63 | 32 | — | — | — | — | |
| 228 | 0 | — | — | — | — | — | — | — | — |
| 20 | 2,233 | 154 | 55 | — | — | — | — | — | |
| 54 | 4,167 | 785 | 230 | 65 | — | — | — | — | |
| 229 | 0 | — | — | — | — | — | — | — | — |
| 20 | 810 | 40 | — | — | — | — | — | — | |
| 54 | 1,473 | 247 | 90 | 46 | — | — | — | — | |
| 236 | 0 | — | — | — | — | — | — | — | — |
| 20 | 1,499 | 1,327 | 29 | — | — | — | — | — | |
| 54 | 7,769 | 7,895 | 218 | 101 | 32 | — | — | — | |
| 241 | 0 | — | — | — | — | — | — | — | — |
| 20 | 3,379 | 36 | 46 | 40 | — | — | — | — | |
| 54 | 5,061 | 313 | 100 | 79 | 112 | — | — | — | |
| Reference HIV-1+ plasma N16 | Mean | 8,497 | 2,036 | 678 | 413 | 52 | 133 | 26 | — |
a
The reciprocal of the dilution which resulted in 50% neutralization of Env-pseudotyped HIV-1 infectivity on coreceptor-bearing U87.CD4 cells. A dash indicates that <50% neutralization was observed at the 1:25 dilution. Plasma (N16) was from an HIV-1-infected individual and was used as a reference standard to control for assay-to-assay variation.