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Mutants of the RSV Gag polyprotein. The wild-type Gag protein, Pr76gag, is depicted at the top, with the protease-deleted protein (Gag.ΔPR) shown below. Gag-GFP has the gene encoding GFP substituted for the last seven codons of NC and all of the PR sequence (26). The potential NES sequence encompassing residues 219 through 229 of the p10 region is highlighted. Substitution L219A, W222A, V225A, and L229A mutants are depicted below the wild-type sequence. CA, capsid.
