FIG. 4.

Kinetics of NES mutant Gag protein release. Transfected QT6 cells were pulse-labeled for 15 min and then chased with cold medium. Medium samples were collected every 15 min, and the amount of Gag protein present in the medium was expressed relative to the amount of Gag.ΔPR protein synthesized during the 15-min pulse. The amount of wild-type Gag protein released after 2.5 h was standardized to 100% for each assay. Curves represent the average of four to five independent experiments, with standard deviations denoted.