TABLE 2.
ESTs detected by a BLAST search with four different nucleotide sequencesa
| Tissue | No. of hits with nt sequence for:
|
|||
|---|---|---|---|---|
| R-U5 (120 bp)b | U3 (471 bp)b | TM+PPT (715 bp)c | Total | |
| Placenta | 10 | 14 | 8 | 32 |
| Skin | 2 | 3 | 5 | |
| Adrenal gland | 1 | 2 | 4 | 7 |
| Testis | 3 | 3 | ||
| Ovary | 2 | 2 | ||
| Pancreatic islet | 3 | 3 | 6 | |
| Kidney | 3 | 9 | 12 | |
| Brain | 7 | 8 | 1 | 16 |
| Optic nerve | 2 | 2 | ||
| Prostate | 3 | 1 | 4 | |
| Cartilage | 4 | 2 | 6 | |
| Lung | 1 | 1 | 2 | |
| Fetal lung | 1 | 1 | 2 | |
| Fetal liver/spleen | 3 | 6 | 1 | 10 |
| Fetal heart | 1 | 2 | ||
| Fetal retina | 1 | 1 | 2 | |
| Colon | 1 | 1 | ||
| Bone marrow | 1 | 1 | 2 | |
| B cells from germinal center | 1 | 2 | ||
| Alveolar macrophage | 1 | 1 | ||
| Total fetus | 4 | 3 | 7 | |
| Carcinoid | 6 | 6 | ||
| Colon tumor | 5 | 4 | 1 | 10 |
| Colon adenocarcinoma | 1 | 1 | ||
| Gastric carcinoma | 2 | 3 | 5 | |
| Endometrial adenocarcinoma | 1 | 1 | ||
| Uterus tumor | 1 | 1 | 2 | |
| Cervical tumor | 1 | 2 | 3 | |
| Kidney tumor | 2 | 2 | ||
| Oligodendroglioma | 5 | 1 | 1 | 7 |
| Neuroblastoma | 1 | 4 | 5 | |
| Leiomyosarcoma | 1 | 1 | ||
| Total | 56 | 86 | 25 | 167 |
a
Hits with a score of more than 46 and a percent identity to ERV3 7q11 of more than 95% are shown. Searches with gag and pol sequences did not yield any ERV3 7q11 specific hits (not shown).
b
An LTR consists of two unique sequences (U3 and U5) and one repeat sequence (R).
c
TM, transmembrane protein nucleotide sequence; PPT, polypurine tract.