FIG. 1.

Viral titers of CVB3(T7) in the heart and pancreas compared with those of the single mutants KR and EG and the combined mutant KR/EG. On days 3, 7, and 10 postinfection, hearts and pancreases were harvested from male A/J mice that had been inoculated with either the wt or a mutant virus. The titers of virus in the heart were about 20-fold lower for the KR mutant and 100-fold lower for both the EG and KR/EG mutants than for the wt strain on day 3. None of the mutant viruses were detected on days 7 and 10, while the mean titer of the wt CVB3(T7) strain was highest on day 7 and residual virus was still detected on day 10. In the pancreas, the titers of all of the mutants were not significantly different from that of the wt on day 3 p.i., but the mutant viruses were more rapidly eliminated than the wt strain.