TABLE 2.
Efficacy of preimmunization with triple mutants on subsequent challenge with a lethal dose of CVB3(T7)
| Immunizing virus | Dose inoculated (PFU) | Morbidity after i.p. inoculation | Neutralizing antibody titer in pooled sera on day 21 | Response to the challenge of 1 × 106 PFU of CVB3(T7)
|
|||||
|---|---|---|---|---|---|---|---|---|---|
| Viral titers at indicated day (d)
|
Antibody titer in sera at day
|
Morbidity or mortality | |||||||
| Serum d 1 (PFU/ml) | Heart d 3 (PFU/g) | Pancreas d 3 (PFU/g) | 7 | 10 | |||||
| PBS | PBS | 0/16 | NDa | 1.1 × 106 | 1.69 × 108 | 5.87 × 108 | 1: 8,000 | NAb | 16/16 |
| CVB3(KR/EG/DE) | 1 × 105 | 0/16 | 1: 8,000 | ND | ND | ND | 1: 96,000 | 1: 128,000 | 0/16 |
| CVB3(KR/EG/PM) | 1 × 105 | 0/16 | 1: 6,000 | ND | ND | ND | 1: 64,000 | >1: 128,000 | 0/16 |
a
ND, not detectable.
b
NA, not available.