FIG. 8.

Colocalization of viral glycoproteins with capsids and TGN. 143B cells were infected with wild-type K26GFP virus (in green) to allow the three-color staining. Cells were incubated for 7 h at 37°C and chased for 6 h at 20°C. They were then fixed and stained with antibodies specific for the TGN (blue) and against viral glycoproteins gE (A), gH (B), or gI (C) in red. It should be noted that the three-color staining prohibited us from staining the nuclei and hence distinguish the capsids still trapped in the nucleus. The arrows show the site of partial colocalization of the capsids with both the TGN46 and glycoproteins (white labeling).