TABLE 1.
SIV RNA and DNA in tissues of rhesus monkeys inoculated intravaginally with SIVmac251 or AT-inactivated SIVmac239 and in uninfected animals
| Animal no. and inoculum | Time of necropsy (days p.i.) | Plasma vRNAe | Genital tracta
|
Genital LNsb
|
Systemic lymphoid tissuesc
|
Intestinal tractd
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| VRNAf | VDNAg | MS RNAh | vRNA | vDNA | MS RNA | vRNA | vDNA | MS RNA | vRNA | VDNA | |||
| SIVmac 251i | |||||||||||||
| 29699 | 2 h | <125 | 2.0 × 103 | + | − | ND | ND | ND | ND | ND | ND | ND | ND |
| 23053 | 2 h | <125 | 2.0 × 103 | − | − | ND | ND | ND | ND | ND | ND | ND | ND |
| 25103 | 1 | <125 | 5.0 × 103 | + | − | ND | ND | ND | ND | ND | ND | ND | ND |
| 26937 | 1 | <125 | + | − | − | − | − | − | − | − | − | ND | |
| 31426 | 1 | <125 | 4.0 × 103 | − | − | − | − | − | − | − | − | − | ND |
| 31937 | 1 | <125 | 1.0 × 104 | 8.0 × 104 | − | − | − | − | − | − | − | − | ND |
| 30127 | 1 | <125 | 4.1 × 102 | − | − | − | − | − | 3 × 102 | − | − | − | ND |
| 31529 | 1 | <125 | − | − | − | − | − | − | − | + | 3 × 102 | ND | |
| 30201 | 2 | <125 | + | − | − | + | − | − | − | − | 7 × 102 | − | |
| 28018 | 3 | <125 | − | − | − | − | − | 1.0 × 103 | − | + | − | ND | |
| 28420 | 3 | <125 | 9.0 × 103 | + | − | − | − | − | − | − | − | − | ND |
| 31373 | 4 | <125 | 3.4 × 102 | 3.5 × 105 | − | − | + | − | − | 3.6 × 102 | − | − | 7.2 × 102 |
| 31433 | 4 | <125 | − | − | − | − | − | − | − | + | − | ND | |
| 31385 | 4 | 1.5 × 103 | − | − | − | − | − | − | − | − | − | − | |
| 34443 | 5 | <125 | − | − | − | − | − | − | 8.5 × 103 | − | − | 2.1 × 103 | |
| 30991 | 6 | 2.3 × 104 | 9.0 × 103 | 1.9 × 103 | − | 1.0 × 105 | 5.0 × 103 | 2.0 × 104 | 5.0 × 103 | ND | 2.0 × 102 | + | |
| 31523 | 6 | 7.3 × 104 | 4.0 × 104 | 2.9 × 104 | − | 8.0 × 104 | + | 4.8 × 104 | + | ND | 1.0 × 103 | 5.0 × 103 | |
| 26222 | 7 | 2.6 × 106 | 9.0 × 105 | + | + | 3.0 × 105 | + | + | 3.4 × 106 | + | + | 1.0 × 105 | ND |
| 34498 | 8 | 2.5 × 106 | 3.2 × 105 | + | + | 1.0 × 105 | + | + | 2.0 × 105 | + | + | 1.0 × 103 | + |
| 28013 | 9 | 2.5 × 106 | 6.0 × 105 | + | + | 2.0 × 106 | + | + | 7.0 × 105 | + | + | 7.0 × 105 | ND |
| 24818 | 10 | 9.9 × 106 | 5.0 × 105 | + | ND | 7.0 × 105 | + | − | 2.0 × 107 | + | − | 1.0 × 106 | + |
| SIVmac AT-2j | |||||||||||||
| 27438 | 1 | <125 | 1.0 × 104 | − | ND | − | − | ND | 2.5 × 102 | − | ND | − | − |
| 27776 | 2 | <125 | − | − | ND | − | − | ND | − | − | ND | − | − |
| 32109 | 3 | <125 | − | − | ND | − | − | ND | − | − | ND | − | − |
| 31098 | 4 | <125 | − | − | ND | − | − | ND | − | − | ND | − | − |
| Uninfectedk | |||||||||||||
| 34572 | 1 | ND | − | ND | ND | − | ND | ND | − | ND | − | ND | |
| 34550 | 1 | ND | − | ND | ND | − | ND | ND | 170 | ND | 800 | ND | |
| 31931 | 1 | ND | − | ND | ND | − | ND | ND | 130 | ND | 180 | ND | |
The genital tract includes three sites in the vagina and one site in the cervix. Results from the tissue samples with the highest vRNA levels are shown.
Genital lymph nodes are the iliac and obturator LNs. Results from the tissue samples with the highest vRNA levels are shown.
Systemic lymphoid tissues are the axillary, inguinal, and mesenteric LNs and the spleen. Results from the tissue samples with the highest vRNA levels are shown.
The intestinal tract is the jejunum, ileum, and colon. Results from the tissue samples with the highest vRNA levels are shown.
SIV RNA copies/μl plasma (125 copies is the lower limit of detection) (see Materials and Methods).
SIV RNA copies/μg of tissue RNA (see Materials and Methods).
Positive (+) or negative (−) detection of SIV DNA by nested PCR or number of SIV DNA copies/μg of tissue RNA by real-time PCR (see Materials and Methods).
Multiply spliced SIV RNA copies/μg of tissue RNA (see Materials and Methods). ND, not done.
Age-matched female rhesus monkeys inoculated intravaginally with SIVmac239 (105 TCID50/ml and 109 vRNA copies/ml).
Age-matched female rhesus monkeys inoculated intravaginally with AT-2-inactivated SIVmac239 (0 TCID50/ml and 1010 vRNA copies/ml).
Age-matched female rhesus monkeys that were never exposed to SIV.