Fig 3. Prime and prime boost immunization with Ad35.HSV.gD2 induces HSV-2 specific IFNγ responses.
Female BALB/c mice were immunized as described in Fig 1. Cellular immune responses were assessed by IFN-γ ELISpot on splenocytes harvested from BALB/c mice (T = 42d). (a) Ad35.HSV.gD2 splenocytes were stimulated with a gD2 peptide pool overnight in R10 medium while (b) CJ2-gD2 splenocytes were stimulated in R0 medium with a gD2 peptide pool, gB2 peptide pools (gB-1, gB-2 and gB-3), ICP27 (13-mer: AFLTGADRSVRLA) and VP11/12 (9-mer: HGPSLYRTF). Results are depicted as spot forming units (SFU)/106 splenocytes. Dotted lines indicate the background of the assay (95th percentile of medium stimulated samples) and the horizontal lines depict the geometric mean response per group. Across dose statistical comparison between the one- and 2-dose regimens was performed by ANOVA. Results of statistical analysis are depicted by asterisks: ** p<0.01.