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. 2024 Dec 31;19(12):e0309979. doi: 10.1371/journal.pone.0309979

Fig 3. Overexpression of miR-1247-3p promoted apoptosis and inhibited resistance in drug-resistant cells.

Fig 3

(A) HCT8/5-Fu and HCT8/DDP cells transfected with miR-1247-3p mimics or negative control. (B) An MTT assay was used to measure the proliferation of HCT8/5-Fu and HCT8/DDP cells after transfection. (C) and (D) Flow cytometry was used to analyze cell cycle and apoptosis. (E) Western blotting was used to detect the expression of apoptosis-related proteins in HCT8/5-Fu and HCT8/DDP cells after transfection. (F) An MTT assay was used to evaluate the sensitivity of HCT8/5-Fu and HCT8/DDP cells to chemotherapeutic drugs after transfection. *P<0.05, **P<0.01, ***P<0.001. miR, microRNA; CRC, colorectal cancer; 5-Fu, 5-fluorouracil; DDP, cisplatin.