FIG. 2.

Induction of CNTFRα expression by RPV/UT infection and NS protein transduction in C58(NT)D cells. C58(NT)D cells were infected with RPV/UT at an MOI of 1 for 48 h. Semiquantitative RT-PCR (A) and Western blotting analysis (B) for CNTFRα and NS-1 were performed with total RNA and cell lysate isolated from infected cells at the indicated time points. Semiquantitative RT-PCR (C) and Western blotting analysis (D) for CNTFRα were performed with cell lysate from C58(NT)D cells infected with NS/EGFP or EGFP retrovirus at an MOI of 3. GAPDH and actin served as internal controls.