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. 2005 Jul;79(14):8886–8893. doi: 10.1128/JVI.79.14.8886-8893.2005

FIG. 4.

FIG. 4.

Epigenetic regulation of CNTFRα expression. (A) Semiquantitative RT-PCR was performed with total RNA extracted from C58(NT)D cells treated with 5-azacytidine for 3 and 6 days. Semiquantitative RT-PCR (B) and Western blotting analysis (C top) with cell lysate from C58(NT)D cells treated with trichostatin A (TSA) and sodium butyrate (NaB) at the indicated concentrations for 12 h using specific primer and antibody for CNTFRα. (C, bottom) Time course experiment of CNTFRα gene expression induced by trichostatin A (100 ng/ml) and sodium butyrate (5 mM) treatment in C58(NT)D cells. GAPDH and actin served as internal controls.