FIG. 9.
Localization of CD8+ T cells and apoptosis in 17 N/H-infected trigeminal ganglia. BALB/c mice were infected by corneal scarification with 2.5 × 104 PFU of 17 N/H virus in each eye. At 30 days postinfection, mice were sacrificed and trigeminal ganglia were extracted and sectioned for slides. Slides were stained for CD8a antigen and apoptosis using TUNEL. Sections were viewed by light microscopy (A), UV microscopy using a rhodamine filter to view CD8a staining (B), and UV microscopy using a fluorescein filter to view TUNEL staining (C). (D) Fields B and C were merged for colocalization of CD8 cells and apoptosis.