FIG. 3.

Chromosome-bound E2 proteins colocalize with Brd4 on mitotic chromosomes. The colocalization of E2 and Brd4 on mitotic chromosomes was examined in cells expressing the mutated E2 proteins A1, K342, and F3. The E2 protein, as detected by a FITC-labeled antibody, is shown in green; the Brd4 protein, as detected by a Texas Red-labeled antibody, is shown in red; and the colocalization of these proteins appears yellow in the merged images. Cellular DNA was detected by DAPI staining and is shown in blue.