TABLE 1.

Phenotypes of E2 proteins used for this study^a

E2 protein	Mutation(s)	Mitotic chromosome association	Brd4 interaction in vitro	Transcriptional regulationb	Transient replication activity
E2-TA	None	+	+	+++	+
E2-TR	None	−	−	−	−
A1	E2A, E6A, E13A, E20A	+	+	+++	−
K342	R342K	+	+c	−	−
F3	R37A, I73A	−	−	+	+

^aData were obtained from references 3, 4, and 54.

^bTranscriptional regulation is expressed as a percentage of the wild-type E2 activity, as follows: +++, <60% activity; ++, 30 to 60% activity; +, 10 to 30% activity; −, no activity.

^cSpindler and McBride, unpublished observation.