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. 2005 Jul;25(14):6123–6139. doi: 10.1128/MCB.25.14.6123-6139.2005

FIG. 5.

FIG. 5.

Loss of UBP8 does not result in increased histone H3 Lys4 and Lys79 methylation and decreased Sir3p binding at telomeres. In vivo cross-linking analysis was performed as described in the legend of Fig. 4 using stains UCC6389 (wild type), UCC6392 (ubp8Δ), UCC6390 (ubp10Δ), UCC6393 (ubp10Δ ubp8Δ), and UCC6391 (sir4Δ). (A) Representative example of Vistra Green-stained PCR amplifications. (B to D) Quantitative analysis of data in shown in panel A was performed identically as described in the legend of Fig. 4B to D. (B) Relative increase in H3 Lys4 trimethylation at telomere VIR and GAL1. (C) Relative increase in H3 Lys79 methylation at telomere VIR and GAL1. (D) Relative decrease in Sir3p binding at telomere VIR. (E) Ubp8p does not function in telomeric silencing. Overnight, saturated cultures of yeast strains UCC6422 (wild type), UCC6423 (ubp10Δ), UCC6424 (sir4Δ), UCC6425 (ubp8Δ), and UCC6426 (ubp10Δ ubp8Δ), which all carry the URA3 gene located near telomere VIIL (85), were serially diluted and spotted onto YC plates, with or without uracil. Cells were grown at 30°C for 3 days.