FIG. 2.
Role of Swi2p ATPase motifs in generation of superhelical torsion. The ability to generate DNA superhelical torsion was tested on a linearized (AvaI-digested) pXG540 template which contains an inverted [AT]34 repeat. Reaction mixtures containing 0.1 nM of AvaI-linearized pXG540, 1.5 nM SWI/SNF (WT or variant), 3 mM ATP, and 0.15 μg/ml Endo VII were incubated for 4 h. Samples were taken at the denoted times, quenched, deproteinated and electrophoresed on 4% native PAGE gels (as described in Materials and Methods). The data shown are representative of multiple experiments.