TABLE 2.
Multicopy suppression of TBP spt16 synthetic lethalitya
| Strain | TBP mutant | Spt16 mutant | Effect with plasmid
|
||
|---|---|---|---|---|---|
| YEp-TFIIA | YEp-TFIIB | YEp-NHP6A | |||
| DY9446 | TBP(E93G) | spt16-2 | Suppression | No effect | No effect |
| DY9447 | TBP(E93G) | spt16-11 | Suppression | No effect | Inhibition |
| DY9450 | TBP(E93G) | spt16-16a | Suppression | No effect | No effect |
| DY9448 | TBP(E93G) | spt16-22 | Suppression | No effect | Inhibition |
| DY9449 | TBP(E93G) | spt16-24 | Suppression | No effect | No effect |
| DY9451 | TBP(K133L,K145L) | spt16-16a | Inhibition | No effect | No effect |
| DY9452 | TBP(G147W) | spt16-2 | Suppression | No effect | No effect |
| DY9453 | TBP(G147W) | spt16-9a | Suppression | No effect | No effect |
| DY8970 | TBP(G147W) | spt16-12 | Suppression | ND | No effect |
| DY8969 | TBP(G147W) | spt16-16a | Suppression | Suppression | No effect |
| DY8971 | TBP(G147W) | spt16-22 | Suppression | No effect | Inhibition |
| DY8972 | TBP(G147W) | spt16-24 | Suppression | ND | No effect |
| DY9456 | TBP(L172P) | spt16-11 | No effect | No effect | No effect |
| DY9454 | TBP(F237L) | spt16-16a | No effect | No effect | No effect |
a
Strain DY8552, with the genes for both TBP and Spt16 deleted but with the YCp-URA3-TBP-Spt16 plasmid, was transformed with the appropriate YCp-TRP1 plasmids with TBP mutants and YCp-LEU2 plasmids with Spt16 mutants and then passed over 5-FOA at 25°C to isolate the indicated strain without the wild-type TBP and Spt16 genes. These strains were then transformed with the YEp-URA3 multicopy plasmids with TFIIA, TFIIB, or NHP6A, or the vector control, and growth was assessed after 3 days at 33°C on selective medium. ND, not done.