FIG. 2.

Effect of TBH on rat GCLC promoter activity in WT, F1, and F2 cells. WT, F1, and F2 cells were transiently transfected with the rat GCLC promoter-luciferase construct −595/+2-LUC (labeled GCLC-LUC) or pGL-3 enhancer vector and treated with TBH (60 μM for 8 h) or vehicle control (DMSO) as described in Materials and Methods. Results represent the mean ± SEM from three independent experiments performed in triplicate. Data are expressed as relative luciferase activity to that of pGL-3 enhancer vector control in WT cells, which is assigned a value of 1.0. *, P < 0.05 versus WT GCLC-LUC; †, P < 0.05 versus respective control and WT GCLC-LUC plus TBH (ANOVA followed by Fisher's test).