FIG. 4.

Steady-state protein levels of the NF-κB family members in WT, F1, and F2 cells. Total cell lysates (40 μg/lane) from WT, F1, and F2 cells were subjected to Western blot analysis using anti-p50, p65, RelB, and c-Rel antibodies as described in Materials and Methods. The same membranes were stripped and probed with antibodies against actin to ensure equal protein loading. The right panels show densitometric changes expressed as percentages of WT. *, P < 0.05 versus WT. Representative blots are shown.